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IVF Procedures

Follow the ET procedures Step 1 to 4, except as follows:

At procedure 3

ALL IVF calves MUST be DNA verified PRIOR to registering the calves.

Imported Embryo's

Please discuss these with the registrar if you are considering importing embryo’s.

NOTE: ALL registrations, in this case, are sent in to Federal Office on paper.

Telephone Holstein Australia on 03 9835 7600 to speak to a registrar.

Buying and Selling Embryo’s

(Frozen and/or recipient cows in calf)

1. The seller (Vendor) is required to submit the Holstein Australia’s “DBC form” for the transfer of ownership of an embryo or recipient in calf.

Information required on this form:

Buyer’s details

Sale Date

Donor Dam’s Id

Sire details

Recovery date of Flush (Important)

Number of embryo’s sold

Recipient dam details or Frozen Straw Ids

Send this form to Holstein Australia, PO Box 489, Hawthorn East, 3122

It is important to send in this form as soon as possible after the sale of an embryo in order to have these details available for the new owner to register the impending calf.

How to get a DNA Test

Ring Holstein Australia to request a DNA testing kit. (Ph: 03 9835 7600) This will be sent to you.

The DNA testing kit comprises the following:

# A sample card in a plastic sealable bag

# A label identifying the animal you are going to be testing.

# A letter of request to DNA Test your animal

One DNA testing kit is provided for each animal requested. (ie. If you are testing 9 animals, 9 kits will be sent out)

Follow the instructions on the back of the card. Attach hair samples (including hair root) and label.

Place the card (with hair sample and matching label attached) into the clear plastic bag.

RETURN the sample/s to

ET Registration, Holstein Australia, PO Box 489, HAWTHORN BC, 3122

(Note: Holstein Australia requires that all samples are sent through the Federal Office in order to receive the discount from our service provider.)

Holstein Australia will forward the sample/s to Queensland University. When results are received and finalised, charges will be applied to your member account and you will receive a copy of the results from HA. Price structure for DNA Testing

Protocol For Collection Of Hair Samples For DNA Analysis By Queensland University

DNA is contained in the root or follicle of a hair, not in the shaft itself. Therefore it is essential that the hair follicle is attached to each plucked hair being submitted for DNA analysis.

SHORT, FINE BODY HAIRS ARE NOT SUITABLE FOR DNA ANALYSIS. ONLY THICK COARSE, THICK TAIL HAIRS WILL BE ACCEPTED.

Collect long thick hairs from the switch or brush of the tail.

It is essential that hairs submitted for DNA analysis are thoroughly dry.

Collect a minimum of 20 hairs from the tail of the animal to be tested. Pluck the hairs from the switch or brush of the tail, DO NOT CUT THE HAIRS. (You could wrap the hair around a pencil and pull.)

Ensure that each extracted hair has a follicle (bulbous hair root) attached.

Place all of the hairs from one animal onto the card supplied with the DNA kit and affix with the supplied label, as instructed on the back of the card. Place the card back into the snap-lock plastic bag (provided when you request your DNA test kit). Do not place the hairs directly into envelopes.

Place the labelled samples that are in plastic bags into an envelope. Post the sample(s) to Holstein Australia Federal Office.

Q. What do I do if my cow does not have a tail?

If your cow does not have a tail you can collect from inside the ear, or perhaps even under the belly – just as long as you do not collect short, fine body hair.

Price Structure for DNA Testing

Fees will be charged to the member account after the testing has been completed.

Labelled DNA Test samples are to be forwarded to Holstein Australia Federal Office for processing.

ALL Prices are GST Inclusive.

Lodgement Fee, ABC (Flush) Form         $22.00

Lodgement Fee, DBC Form                    $22.00

DNA Type from Hair sample                    $33.00

Parentage determination/verification         $5.50

If a full DNA type is unable to resolve parentage then additional tests are available at a cost of $7.70 per test per animal.

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Glossary

ABC Form Flush Form. Complete this form to record the details of a flush and identify each embryo recovered from a flush.

AI  Artificial Insemination

DBC Form  An application form to be completed upon sale or export of an embryo, whether implanted or frozen.

DNA Testing  A test provided to record the molecular DNA relevant for use in determining the parents of the animal.

Donor Dam  The biological dam from which embryos’ have been flushed

ET  Embryo Transfer

Federal Office  Holstein Australia, PO Box 489, HAWTHORN BC, 3122

Flush The procedure of recovering one or more embryos after super